Abstract
The MIRAGE guidelines are being developed in response to a critical need in the glycobiology community to clarify glycoanalytic results so that they are more readily evaluated (in terms of their scope and depth) and to facilitate the reproduction of important results in the laboratory. The molecular and biological complexity of the glycosylation process makes thorough reporting of the results of a glycomics experiment a highly challenging endeavor. The resulting data specify the identity and quantity of complex structures, the precise molecular features of which are sometimes inferred using prior knowledge, such as familiarity with a particular biosynthetic mechanism. Specifying the exact methods and assumptions that were used to assign and quantify reported structures allows the interested scientist to appreciate the scope and depth of the analysis. Mass spectrometry (MS) is the most widely used tool for glycomics experiments. The interpretation and reproducibility of MS-based glycomics data depend on comprehensive meta-data describing the instrumentation, instrument setup, and data acquisition protocols. The MIRAGE guidelines for MS-based glycomics have been designed to facilitate the collection and sharing of this critical information in order to assist the glycoanalyst in generating data sets with maximum information content and biological relevance.
Highlights
The increasing importance of glycoscience in modern biology was recently described in the publication Transforming Glycoscience: A Roadmap for the Future, prepared by the U.S National Academy of Sciences (1)
Standardization is required in order to integrate glycomics data that are widely spread among diverse databases and thereby facilitate the development and application of bioinformatic tools for the analysis of these data
The MIRAGE-Mass spectrometry (MS) guidelines have been proposed in order to encourage authors, editors, and reviewers to gather and report all essential information describing a glycomics experiment that is being reported
Summary
The increasing importance of glycoscience in modern biology was recently described in the publication Transforming Glycoscience: A Roadmap for the Future, prepared by the U.S National Academy of Sciences (1). To make it easier for authors to identify appropriate guidelines, a platform project called Minimum Information for Biological and Biomedical Investigations has been developed to provide descriptions for each guideline, including the type of information that is required in order to thoroughly report each particular experiment (5).
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