Abstract
A method is described for the determination of total fatty acids of serum by microtitration. The method is designed to permit also the determination of cholesterol and lipid phosphorus, and of triglycerides by difference. One ml. of serum is sufficient for duplicate determinations of total fatty acids, lipid phosphorus, and cholesterol. The essential steps include extraction of serum lipids, saponification, extraction and microtitration of the liberated fatty acids. A Possible modification which may be useful in the estimation of triglycerides is described. Recoveries of pure fatty acids and triglycerides were 97 per cent complete. Short chain fatty acids and intermediates of carbohydrate metabolism were not detected by this method.
Highlights
Using the above formula, it was determined that the concentration of total fatty acids in the serum was 15.0 meq. per 1
I n the entire range of total fatty acids tested, the mean difference between duplicate determinations was 0.40meq. per 1. f 0.37 meq. per 1
The completeness of recovery of fatty acids was tested by carrying out the entire procedure on solutions containing known concentrations of pure fatty acids or triglyceride.s The material to be tested was dissolved in hexane in known concentrations
Summary
A method is described for the determination of total fatty acids of serum by microtitration. Of serum is sufficient for duplicate determinations of total fatty acids, lipid phosphorus, and cholesterol. The fatty acids occur hydrolysis of serum lipids permits direct estimation of in phospholipids, esters of cholesterol, and in total fatty acids as equivalents. The method is of sufficient nominator, and when determined with cholesterol and simplicity to permit its use as a routine procedure, phospholipids, permits by calculation estimation of yet accurate enough for use as a research tool.
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