Abstract
The Micromorphological Course of Irradiation-Induced Oral Mucositis in Rat Abstract Objectives: To establish an experimental radiation-induced mucositis model in the Sprague-Dawley rat, and to use this model to study the temporal changes in morphology, including invasion by immune cells (polymorphonuclear (PMN) cells and macrophages – both activated M1 macrophages and wound healing M2 macrophages) following irradiation. Materials and methods: Irradiation was given as a single fraction treatment to the entire head using a conventional high-energy linear accelerator (Varian Clinac 2300 C/D). Treatment was in as single fractions of 20 Gy, using 6 MV photons. Morphological changes in irradiated lingual and buccal tissues were assessed using a haematoxylin-eosin staining, while invasion by immune cells was established by immunohistochemistry. Results: A single dose of 20 Gy gave rise to ulcerations and a manifest oral mucositis. Atrophy of the epithelial layer was seen on day 5 in the buccal specimens and on day 7 in the lingual specimens. Regeneration of the epithelial layer was observed day 13 in the buccal specimens and on day 17 in the lingual specimens. A peak influx of PMN cells was observed before a peak of macrophages was seen. The concentration of PMN cells decreased after the acute phase had passed – and was then lower than in control samples. A peak in the influx of general macrophages (ED 1 stain) was observed day 9, and also on day 11 of M2 macrophages (ED 2 stain). Conclusion: An experimental model of irradiation-induced oral mucositis was established in the Sprague-Dawley rat, using a high-energy linear accelerator, which provides a research platform for the study of radiotherapy-induced oral mucositis pathogenesis. A uniform morphological pattern was observed, showing a rapid healing process following irradiation. An influx of PMN cells peaked before the macrophage peak, whereas those peak of M2 macrophages occurred 2 days after the peak of general macrophages.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
