Abstract
The object of this study is to characterize the bacterial community of subgingival plaque of two subjects with generalized aggressive periodontitis (GAgP) pre- and post-treatment. We picked two patients with GAgP and used high-throughput 16S rDNA sequencing. V4 hypervariable region was picked for PCR amplification of subgingival samples. Then, the PCR products were sequenced through Illumina MiSeq platform. One month after therapy, both the clinical features and periodontal parameters improved obviously. Moreover, the composition and structure of subgingival bacterial community changed after initial periodontal therapy. Also, the composition of the subgingival microbiota was highly individualized among different patients. Bacteroidetes, Spirochaetes and Fusobacteria were related to pathogenicity of GAgP while Actinobacteria and Proteobacteria seemed associated with clinical symptoms resolution. In this study, we found the subgingival bacterial community was high in species richness but dominated by a few species or phylotypes, with significant shifts of microbiota that occurred after treatment. This study demonstrated the shift of the subgingival bacterial community before and after treatment by high-throughput 16S rDNA sequencing, and provided a concise method for analysis of microbial community for periodontal diseases.
Highlights
The oral cavity contains a large number of ecological sites that provide surfaces for microbial colonization
Only about half of the subgingival bacterial species or phylotypes are cultivable, which presents an obstacle to fully understand the causal relationship between subgingival bacteria and periodontitis [7]
Many potential pathogens are associated with periodontitis, while traditional culture-dependent methods can not detect all oral microbiomes since most of them have a very little trace or are uncultivable
Summary
The oral cavity contains a large number of ecological sites that provide surfaces for microbial colonization. Studies revealed that the predominant bacteria were normal flora, which were non-pathogenic and helped prevent colonization by exogenous organism, and were considered to be commensal in the oral cavity [1, 2]. Approximately 200 bacterial species have been found in the oral cavity. Only about half of the subgingival bacterial species or phylotypes are cultivable, which presents an obstacle to fully understand the causal relationship between subgingival bacteria and periodontitis [7]. Whether the microbial community shifts before and after periodontal therapy remains unclear. The members of the microbial community are typically determined by tracking phylogenetic markers such as the 16S rDNA
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