The method to detect sIgE to thyroid peroxidase in cases of the patients with autoimmune and allergic diseases

A V Zubkov,L G Butova,N M Rahmatulina,S A Mazurina,N S Kuzmina,N A Sibgatulina,V B Gervazieva

doi:10.36233/0372-9311-2019-3-31-36

Abstract

Aim of this research has been to create a method to detect sIgE to thyroid peroxidase (TPO) in serum of the patients with autoimmune and allergic diseases.Materials and methods. In this research native affine-purified TPO (more the 95% clean, enzyme activity 60±15 of enzyme unit per mg) with immune dominant elements only (confirmed by inhibitory ELISA) has been used. The detection of sIgE in serum, combined with TPO, was carried out with monoclonal antibodies to IgE (8E/4F4) conjugated with horseradish peroxidase.Results. In serum of patients with bronchial asthma, allergic rhinitis and chronic spontaneous urticarial sIgE to TPO has been detected in 18—21% of cases. The healthy people and the patients with autoimmune thyroiditis had no sign of sIgE to TPO in their serum.Conclusion. The detection of sIgE to TPO in cases of patients with chronic spontaneous urticarial and allergic diseases will make possible to perform the early diagnosis of thyroid autoimmune disorders and provide timely and adequate therapy.

Highlights

In serum of patients with bronchial asthma, allergic rhinitis and chronic spontaneous urticarial sIgE to thyroid peroxidase (TPO) has been detected in 18—21% of cases
The detection of sIgE to TPO in cases of patients with chronic spontaneous urticarial and allergic diseases will make possible to perform the early diagnosis of thyroid autoimmune disorders and provide timely and adequate therapy
Ранняя диагностика аутоиммунных процессов в щитовидной железе с использованием метода определения sIgE к ТПО, наряду с IgG-аутоантителами, у больных с иммуно-опосредованными заболеваниями, в том числе при хронической спонтанной крапивницей (ХСК), позволит своевременно диагностировать патологию щитовидной железы и обеспечить адекватную своевременную терапию

Summary

Introduction

МЕТОД ОПРЕДЕЛЕНИЯ sIGE К ТИРЕОПЕРОКСИДАЗЕ В СЫВОРОТКЕ КРОВИ БОЛЬНЫХ С АУТОИММУННЫМИ И АЛЛЕРГИЧЕСКИМИ ЗАБОЛЕВАНИЯМИ Создание метода определения sIgE к тиреопероксидазе (ТПО) в сыворотке крови пациентов с аутоиммунными и аллергическими заболеваниями. В сыворотке крови пациентов с аутоиммунным тиреоидитом (АИТ) и у здоровых лиц sIgE к ТПО не были обнаружены.

Results

Conclusion