Abstract
The metabolism of leukotrienes (B 4, C 4, D 4, and E 4) within human plasma was studied and a simple sample preparation is presented. It was demonstrated that leukotriene E 4 and leukotriene B 4 were stable during incubation at 37° C using the in vitro system. In contrast, leukotriene C 4 was metabolized by γ-glutamyl transpeptidase activities into leukotriene D 4 which was further metabolized by dipeptidase activities of plasma into leukotriene E 4. The transition state inhibitor of γ-glutamyl transpeptidase l-serine-borate decreased the metabolism of leukotriene C 4 in plasma. Dilution of plasma demonstrated that the dipeptidase was more active compared to the γ-glutamyl transpeptidase. The metabolizing activities of plasma were functionally characterized by fractionating the plasma proteins.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
