Abstract
Rat brain soluble fraction contains an enzymatic activity that dephosphorylates inositol 1,4-bisphosphate (Ins(1,4)P 2). We have used anion exchange h.p.l.c. in order to identify the inositol monophosphate product of Ins(1,4)P 2 hydrolysis (i.e. Ins(1)P 1, Ins(4)P 1 or both). When [ 3H]Ins(1,4)P 2 was used as substrate, we obtained an inositol monophosphate isomer that was separated from the co-injected standard [ 3H]Ins(1)P 1. This suggested an Ins(1,4)P 21-phosphatase pathway leading to the production of the inositol 4-monophosphate isomer. The dephosphorylation of [ 32P]Ins(4)P 1 was measured in rat brain, liver and heart soluble fraction and was Li +-sensitive. Chromatography of the soluble fraction of a rat brain homogenate on DEAE-cellulose resolved a monophosphate phosphatase activity that hydrolyzed both [ 3H]Ins(1)P 1 and [4- 32P]Ins(4)P 1 isomers.
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