Abstract
THE METABOLISM OF HYDROCORTISONE BY KIDNEY TISSUE IN VITRO
Highlights
MethodsFresh bovine kidneys were obtained from the abattoir approximately 30 minutes after the death of the animals
In t.his study it has been observed that kidney tissue has the capacity to reduce the C-20 ketone group of hydrocortisone and to cleave oxidatively the side chain with the resultant formation of C-19 compounds, without active met,abolism of the A4-3-ketone group to reduct.ion products of ring A
These findings substantiate the suggestions of Schneider and Horstmann that the metabolism of t.his type of steroid may involve the reduction at C-20 and the ext.ensive enzymatic degradation of the side chain ((6) p. 637)
Summary
Fresh bovine kidneys were obtained from the abattoir approximately 30 minutes after the death of the animals. The fat and connective tissue were removed, and the tissue was ground to a mince by means of a commercial grinder. Of this mince were introduced into each of twenty (1 liter) Erlenmeyer flasks. To the tissue in each flask were added 150 ml. Of calcium-free Krebs-Ringer-phosphate buffer, 0.1 M, pH 7.4, containing 10 ml. To this solution were added 20 ml. Of propylene glycol, after which the ethanol was removed by evaporation under nitrogen at 40”. This propylene glycol solution of steroid was added to a 10 per cent kidney homogenate (prepared with buffer). To eacahincubation flask were added 10 ml. To eacahincubation flask were added 10 ml. of this kidney homogenate the United States Atomic Energy Project, Rochesgrant from the Jane Coffin
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