Abstract

AbstractThe oxygen uptake and metabolism of human spermatozoa has been measured using concentrated suspensions of cells incubated with radioactive fructose in small Warburg flasks. The oxygen uptake was about 14 μl per 10S sperm per hr and represented true respiratory activity since radioactive carbon dioxide was produced as well as lactic acid.The presence of 100 μg of prostaglandin E1 per ml had no effect on oxygen uptake, the amount of fructose metabolised or the amount of lactic acid accumulating.

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