Abstract
The metabolism of 25-hydroxycholecalciferol by isolated chick renal tubules has been investigated by the use of a new chromatographic method employing silica-treated paper as the supporting phase. This method has been found to be superior to Sephadex LH20 column chromatography in resolving the metabolites of 25-hydroxycholecalciferol, particularly in separating 1,25-dyhydroxycholecalciferol from other metabolites. With this method several heretofore undescribed metabolites of 25-hydroxycholecalciferol have been recognized and partially characterized.
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