The metabolic and protein markers of dysfunction of mitochondria in patients with cardio-vascular diseases.

Abstract

The article deals with study concerning content of cytochrome C (CytC) and protein PGC1α (1alpha-co-activator of gammareceptor, activating proliferation of peroxisomes) in blood of patients with pathology of left ventricular outflow tract conjointly with low-molecular metabolites characterizing dysfunction of mitochondria and endothelium. The samples of blood plasma were analyzed taking from healthy persons (n=34), patients with aortic aneurysm (n=69), aortic stenosis (n=25) and without pathology of aorta (n=16). In the group of patients with disturbed blood circulation increasing of level of metabolic markers of dysfunction of mitochondria (lactate, ratio lactate/pyruvate) and endothelium (asymmetric dimethylarginine, total homocysteine) was established. The occurrences of cells death were detected according to increasing of level of CytC in 17% 0f patients. The evaluation of level of intermediates of energy metabolism and simultaneously of protein PGC1α permits detecting at least three degrees of dysfunction of mitochondria depending on stage of its progression: 1) without lactoacidemia but with detection of PGC1α in blood, 2) with lactoacidemia up to 2.2 mM and increased level of PGC1α in blood (more than 61 ng/l), 3) with lactoacidemia and loss of cells capacity to transfer this protein in blood. The application of common metabolic indicators of disorder of function of mitochondria supplemented by determination of level of CytC and PGC1α in blood permits evaluating more comprehensively development of dysfunction of mitochondria in patients with disorders of blood circulation of various genesis.