Abstract
Objective:The gut microbiota contribute otherwise impossible metabolic functions to the human host. Shifts in the relative proportions of gut microbial communities in adults have been correlated with intestinal disease and have been associated with obesity. The aim of this study was to elucidate differences in gut microbial compositions and metabolite concentrations of obese versus normal-weight children.Materials and methods:Fecal samples were obtained from obese (n=15; mean body mass index (BMI) s.d. score=1.95) and normal-weight (n=15; BMI s.d. score=−0.14) Swiss children aged 8–14 years. Composition and diversity of gut microbiota were analyzed by qPCR and temperature gradient gel electrophoresis (TGGE).Results:No significant quantitative differences in gut microbiota communities of obese and normal-weight children were identified. Microbial community profiling by TGGE revealed a high degree of both intra- and intergroup variation. Intergroup comparison of TGGE profiles failed to identify any distinct populations exclusive to either obese or normal-weight children. High-pressure liquid chromatography analysis identified significantly higher (P<0.05) concentrations of short-chain fatty acids (SCFA) butyrate and propionate in obese versus normal-weight children. Significantly lower concentrations of intermediate metabolites were detected in obese children, suggesting exhaustive substrate utilization by obese gut microbiota.Conclusions:Our results indicate that a dysbiosis may be involved in the etiology of childhood obesity. In turn, aberrant and overactive metabolic activity within the intestine could dictate survival or loss of individual microbial communities, leading to the altered population ratios previously identified in adult obesity.
Highlights
The human gut is home to over 1 trillion microbes, collectively referred to as the gut microbiota
The children were classified as obese or normal-weight, based on gender- and age-specific body mass index (BMI) percentiles from the Centers for Disease Control, in which obesity is defined as X95th percentile and normal-weight defined as o85th percentile
The butyrate-producing Roseburia/E. rectale population of Clostridia cluster XIVa was over 1 log higher in both obese and normal-weight children compared with the cluster IV butyrate-producer F. prausnitzii (Table 3)
Summary
The human gut is home to over 1 trillion microbes, collectively referred to as the gut microbiota. The aim of this study was to examine the gut microbial compositions and fecal metabolite concentrations of obese and normal-weight children. Fecal samples were obtained from obese (n 1⁄4 15; mean body mass index (BMI) s.d. score 1⁄4 1.95) and normal-weight (n 1⁄4 15; mean BMI s.d. score 1⁄4 À0.14) Swiss children aged 8–14 years and analyzed for differences in both community composition and metabolite concentrations. Individual microbial populations were quantitatively evaluated by qPCR. Fecal metabolite concentrations were measured using high-pressure liquid chromatography (HPLC)
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