Abstract
Commercial preparations of yeast hexokinase are contaminated with a trace of at least one proteolytic enzyme. This contaminant will digest the hexokinase in the presence of sodium dodecyl sulfate (SDS), and a reliable molecular weight can be obtained by SDS-polyacrylamide gel electrophoresis only when specific steps are taken to prevent this proteolysis. When appropriate precautions are taken, this technique yields a value of about 51,000 for the polypeptide chain molecular weight of yeast hexokinase. A similar value is obtained by gel filtration in 6M guanidine-HCl.
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