Abstract
The meiotic segregation of chromosomes 10 and 12 was analyzed in a male heterozygous for a reciprocal translocation, t(10;12)(q26.1;p13.3), using fluorescence in situ hybridization (FISH). Centromeric specific probes that detect alpha satellite sequences of chromosomes 10 and 12 were used. A total of 10,049 spermatozoa were analyzed. The frequencies of alternate/adjacent 1, adjacent 2, and 3:1 modes of segregation were: 84.25, 10.95%, and 4.42%, respectively. Diploidy was present in 0.23% of spermatozoa. Similar segregation patterns have been reported for this donor by direct karyotyping of sperm cells. FISH is a valuable technique for studying meiotic segregation patterns in that larger samples can be studied in a relatively short time. However, it does not provide information on the full chromosome complement of the spermatozoon.
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