Abstract
Brucellosis is a bacterial zoonotic disease which can be easy to misdiagnose in clinical microbiology laboratories. In the present study, we have tried to improve the current clinical method for detecting Brucella spp. and its antibiotic characteristics. Our method begins with detecting the clinical isolate through traditional biochemical methods and automatic identification systems. Then, we move on to editing the sequence for BLAST allows us to compare 16s rRNA sequences with sequences from other species, allowing the gene level to be determined. Next, the phylogenetic analysis of multiple genetic loci is able to determine the evolutionary relationships between our bacteria strain and those from other locations. Finally, an anti-microbial susceptibility test hones in on the level of antibacterial activity that the bacteria displays. Employing these four steps in concert is extremely effective in identifying rare bacteria. Thus, when attempting to determine the identity of rare bacteria such as Brucella, utilizing these four steps from our research should be highly effective and ultimately prevent further identification errors and misdiagnoses. The standards we have suggested to identify rare bacteria strains is applicable not only to Brucella, but also to other rarely encountered bacteria.
Highlights
Brucellosis is one of the most common zoonotic diseases, with more than 500,000 new cases yearly
After comparison with the brucellosis in the Genbank as reference sequences, the bacterial strain in this study was clustered with the Brucella melitensis strains in a significantly monophyletic branch of the neighboring joining tree
The branch lengths represent the genetic variation between Taiwan Brucella melitensis and strains from other geographic areas
Summary
Brucellosis is one of the most common zoonotic diseases, with more than 500,000 new cases yearly. Its prevalence is more than 10/100,000 population in some endemic areas such as France, Israel, and most of Latin America, the Middle East, northern Africa, and central Asia [1, 2]. The disease is transmitted by consumption of unpasteurized dairy products or by occupational contact with infected animals. In the past 15 years, the epidemiology of human brucellosis has increasingly evolved through tourism and cases of animal brucellosis [2]. Infected objects are the most common cause of laboratory-transmitted infections in laboratory workers [3, 4]. Brucella spp. has been classified in the high risk group of pathogens [5]
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