Abstract
The steady-state concentration of urate in slices and separated tubules of rabbit kidney cortex incubated in an electrolyte- and urate-containing medium has been examined under different conditions. 1. The ratio between the concentration of urate in tubule water and medium (T/M Ur ) under aerobic conditions had a constant value of 0.4 in slices at medium concentrations of urate below 0.10 mM. However, renal accumulation of urate was evident at medium concentrations above 0.15 mM. 2. Addition of probenecid (5 mM) resulted inT/M Ur values for slices of 0.4 under aerobic conditions, irrespective of the medium concentration of urate. 3. T/M Ur values were close to unity during anaerobic incubation of slices and were not affected by the presence of probenecid in the medium under these conditions. 4. The accumulation of urate in tubules was similar to that observed in slices. The uptake of urate approached a maximal value at a medium concentration of 5 mM. 5. Low concentrations of probenecid, 2,4-dinitrophenol,p-aminohippurate, and hippurate competitively inhibited the uptake of urate in tubules at high medium concentrations of urate. 6. It is concluded that tubular accumulation of urate is a consequence of tubular secretion, whereas extrusion of urate from tubules may be part of the reabsorptive process for urate in the functioning kidney.
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