Abstract
Highly purified sucrase accepts as a substrate α-anomers within D-glucopyranosides. Hydrolysis of sucrose and palatinose processds with the net retention of configuration at the C-1 of D-glucopyranose. The fission of the glycosidic bond takes place between the C-1 of the D-glucose ring and the glycosidic oxygen; therefore D-fructose is released as β-D-fructofuranose from sucrose. During hydrolysis, transglycosylation takes place and a few oligosaccharides are formed and in the presence of methanol α-methyl-D-glucopyranoside is also produced. Non-stoichiometric amounts of D-glucose and D-fructose are produced from sucrose, turanose and palatinose and less D-glucose than expected is present. Formation of sucrose from D-glucose and D-fructose was not observed.
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