Abstract
Human bank blood erythrocytes were given hemolyzing (16 to 50 kR) exposures of60 Co gamma rays, suspended in various media, and maintained at 5°C for 20 hours. Hemolysis occurred in isotonic NaCl but was prevented or reduced in isotonic choline chloride and in hypertonic NaCl or KCl. Prior to hemolysis in isotonic NaCl, the cells rapidly lost potassium and accumulated sodium. Although irradiated suspensions of cells in choline chloride did not hemolyze, they lost potassium. 2-Mercaptoethylguanidine (MEG) and reduced glutathione (GSH), which does not enter cells, reduced hemolysis and potassium loss when added to the suspensions before irradiation. Radiation caused a marked decrease in membrane sulfhydryl groups. Blockage of membrane sulfhydryl groups by p-chloromercuribenzoate (FCMB) and p-chloromercuribenzene sulfonate (PCMBS), which does not enter the cells, mimicked radiation by causing potassium loss and hemolysis. PCMBS-induced hemolysis was prevented by suspension of the cells in isotonic choline ch...
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