Abstract
To investigate the relationship between miR-410-3p, miR-34c and nasopharyngeal carcinoma development, we detected the expression of miR-410-3p and miR-34c in nasopharyngeal carcinoma tissues and evaluate its clinical value as a molecular marker for predicting the prognosis in patients with nasopharyngeal carcinoma through clinical case study. To identify the role and mechanism of miR-410-3p and miR-34c in nasopharyngeal carcinoma development and progression. The expression of miR-410-3p and miR-34cin 300 cases of nasopharyngeal carcinoma tissues and 30 cases of paired adjacent normal breast tissues was detected by RT-qPCR. The paired t-test was used to compare the differences of miR-410-3pand miR-34c levels between the nasopharyngeal carcinoma and normal groups. The Chi-square test was used to compare the differences between miR-410-3p and miR-34c expression and clinicopathological factors. The Kaplan-Meier survival curve was used to analyze the relationship between miR-410-3p and miR-34c expression and 5-year overall survival (OS). The Cox proportional hazards regression model was used to evaluate the prognostic value. The results were validated by TCGA database. The expression of miR-410-3p was down-regulated in nasopharyngeal carcinoma tissues compared with that of paired normal tissues (P<0.001). The patients with lower miR-410-3p expression have a higher ratio of positive lymph node status (P=0.039) and a poorer 5-year disease-free survival (P=0.001) and 5-year overall survival (P = 0.002). The expression of mi R-34c was down-regulated in nasopharyngeal carcinoma tissues compared with that of paired normal tissues (P<0.001). Up-regulation of the miR-34c inhibited the viability of paired normal tissues (P<0. 01), but there was no significant change in migration of the paired normal tissues. Downregulation of mi R-34c promoted the viability and migration of paired normal tissues (P<0. 05). The expression of miR-410-3p and miR-34c levels are predictors for the OS in patients with nasopharyngeal carcinoma. The expression of miR-410-3p and miR-34c are molecular markers of early nasopharyngeal carcinoma metastasis and an independent prognostic biomarker for patients with nasopharyngeal carcinoma.
Highlights
Nasopharyngeal carcinoma (Nasopharyngeal carcinoma NPC) is a common malignant tumor[1]
RT-QPCR was used to detect the expression levels of Mir-4103p and Mir-34c in 30 primary nasopharyngeal carcinoma tissues and their matching para-carcinoma normal tissues, and it was found that 63.3% of the patients, The expression of Mir-410-3p and Mir-34c was significantly down-regulated (> 25%), and there was a statistical difference between them (P < 0.001) (Figure 1)
The expression levels of Mir-410-3p and Mir-34c in 300 primary nasopharyngeal carcinoma tissues were detected by RT-QPCR
Summary
Nasopharyngeal carcinoma (Nasopharyngeal carcinoma NPC) is a common malignant tumor[1]. Due to drug resistance and other conditions of the tumor, 19%-29% of patients were accompanied by cervical lymph node and/or distant metastasis after treatment, with significantly increased risk of metastasis and recurrence, and sharply increased mortality[3,4,5,6,7]. Due to the limitation of research progress, there is currently a lack of highly effective and low-toxicity targeted drugs and new therapeutic methods, and there are still some patients with nasopharyngeal carcinoma who cannot be cured due to distant metastasis. Improving the early diagnosis and prognosis of nasopharyngeal carcinoma is an effective way to improve the overall treatment level of nasopharyngeal carcinoma
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