The mechanism of bone marrow-derived endothelial progenitor cells in the treatment of peripheral neuropathy of diabetic rats

Abstract

Objective To explore the mechanism of bone marrow-derived endothelial progenitor cells(EPCs)in the treatment of peripheral neuropathy of diabetic rats. Methods Rats with diabetic peripheral neuropathy(DPN)were induced by streptozotocin(60 mg/kg). Male SD rats were divided into normal control group(NC group), DPN group, DPN+ saline group(DPN+ S group), and DPN+ EPCs group. Sciatic nerve motor nerve conduction velocity(MNCV)was measured. The expressions of NF-κB and myelin basic protein(MBP)in sciatic nerve were detected by immunohistochemistry. Results Compared with DPN group, the expression of NF-κB was reduced in the sciatic nerve of DPN+ EPCs group, while the expression of NF-κB was increased in the sciatic nerve of DPN+ S group. There was no statistical difference in the expression of MBP between DPN and DPN+ EPCs groups. Compared with DPN+ S group, the expression of MBP was higher in DPN+ EPCs group. Conclusion Transplantation of EPCs inhibits the expression of NF-κB and increases the expression of MBP, which might be conducive to the repairs of nerve injury. (Chin J Endocrinol Metab, 2018, 34: 312-317) Key words: Endothelial progenitor cells; Diabetic rats; Diabetic peripheral neuropathy