Abstract
The measurement of cytosolic calcium concentration, [Ca 2+] i, in the higher plant cells has proved difficult due to the negligible uptake of [Ca 2+] i indicator. The uptake of the fluorescent [Ca 2+] i indicator, quin 2, as its permeant ester, quin 2/AM, proved unsuccessful when used with plant cells and cell protoplasts. However, electrically induced membrane permeabilisation, electroporation, has allowed quin 2 uptake into mung bean root protoplasts to 10 −4 M. Resting [Ca 2+] was measured as 171 ± 41 nM. The loaded quin 2 was responsive to changes in the Ca 2+ environment of the protoplasts, indicating a reversible fall in [Ca 2+] to 17% of the initial value, over 10 min, on incubation with EGTA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
