Abstract
A method is described which measures the four main prostaglandins of human semen (PGE 1, E 2, 19-hydroxy PGE 1, and 19-hydroxy PGE 2). For routine measurements E 1 and E 2 are measured together as are 19-OH E 1 and 19-OH E 2. These are measured by forming oximes in aqueous solution. extraction, methylation and trimethyl silylation followed by gas chromatography. The method has sufficient sensitivity to measure the levels found in the majority of semen samples. The normal range in men with proven fertility was 90 to 260 μg/ml of 19-hydroxy Es and 30–200 μg/ml of Es.
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