Abstract
This report describes the techniques we have developed to record changes in intracellular calcium concentration which take place during action potentials in the cell body of rat dorsal root ganglion neurones in vitro. The photoprotein, aequorin, was microinjected into the cell body of individual sensory neurones and light output from Ca 2+-activated aequorin molecules was recorded with a photomultiplier tube attached to a modified inverted microscope. Aspects of the technology outlined here include: cell culture methods; a flow chamber for electrophysiological experiments on cell culture preparations; modifications to our inverted microscope: use of 150 mM KCl-filled microelectrodes; and an electronic device for processing the photomultiplier output. Some preliminary results are presented.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
