Abstract

Cholesterol is a major constituent of cell membranes and has many important cell functions. The maximum solubility of cholesterol in a lipid bilayer is the highest mole fraction of cholesterol that can be incorporated into a lipid bilayer before cholesterol crystals precipitate. The maximum solubility can provide valuable information about cholesterol-phospholipid interaction. In this study, the maximum solubility of cholesterol in mixtures of POPE/POPC lipid bilayer has been investigated systematically using a cholesterol oxidase (COD) reaction rate assay.

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