The Marine Catenovulum agarivorans MNH15 and Dextranase: Removing Dental Plaque.

Lai Lai,Lyu Lyu,Wang Wang,Liu Liu,Deng Deng,Tian Tian,Feng Feng

doi:10.3390/md17100592

Abstract

Dextranase, a hydrolase that specifically hydrolyzes α-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of Catenovulum agarivorans MNH15 was screened from marine samples. When the temperature, initial pH, NaCl concentration, and inducer concentration were 30 °C, 8.0, 5 g/L, and 8 g/L, respectively, it yielded more dextranase. The molecular weight of the dextranase was approximately 110 kDa. The maximum enzyme activity was achieved at 40 °C and a pH of 8.0. The enzyme was stable at 30 °C and a pH of 5–9. The metal ion Sr2+ enhanced its activity, whereas NH4+, Co2+, Cu2+, and Li+ had the opposite effect. The dextranase effectively inhibited the formation of biofilm by Streptococcus mutans. Moreover, sodium fluoride, xylitol, and sodium benzoate, all used in dental care products, had no significant effect on dextranase activity. In addition, high-performance liquid chromatography (HPLC) showed that dextran was mainly hydrolyzed to glucose, maltose, and maltoheptaose. The results indicated that dextranase has high application potential in dental products such as toothpaste and mouthwash.

Highlights

Dextran is a water-soluble polysaccharide linked by α-1,6-glucosidic bonds, which can be synthesized by bacteria such as Leuconostoc mesenteroides, Streptococcus, and Rhizopus [1,2,3].Water-soluble exopolysaccharides of dextran mainly play a role in plaque formation [4,5]
The activity of dextranase increased to 128.71% under the treatment of 5 mM SrCl2, which was consistent with the results reported by Wu et al [17,39,45,46]
The dextranase-producing MNH15 strain of C. agarivorans was screened from marine samples

Summary

Introduction

Dextran is a water-soluble polysaccharide linked by α-1,6-glucosidic bonds, which can be synthesized by bacteria such as Leuconostoc mesenteroides, Streptococcus, and Rhizopus [1,2,3]. Water-soluble exopolysaccharides of dextran mainly play a role in plaque formation [4,5]. It has been proven that dextranase can hydrolase dextran safety and effectively. Dextranase (α-1,6-d-glucan-6-glucanohydrolase, E.C.3.2.1.11), an inducible enzyme, is a hydrolase that catalyzes the hydrolysis of α-1,6-glucosidic bonds in dextran [12,13,14]. Dextranase can hydrolyze dextran in sugar juice, which can reduce its viscosity and increase the yield of sucrose [8,9,16]. Dextranase can degrade the high molecular weight of dextran into a

Methods

Discussion

Conclusion