Abstract
An investigation has been made of the poor penetration obtained by the Marchi method. Use of surface tension depressants or perfusion technics showed no marked improvement. Optimum results cannot be achieved with slices of tissue above 3 mm. in thickness. Another cause of faulty staining is the failure to maintain an adequate strength of OsO4 in the Marchi mixture. The concentration of this chemical should never be allowed to drop below 0.25%. A chemical method for determining the amount of OsO4 present in the staining mixture and based upon the Alvarez test is described. This method can also be utilized to reclaim partially exhausted staining solutions. Remarks are included upon the KIO3 method of Busch.
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