The management of bone defect using long non-coding RNA as a potential biomarker for regulating the osteogenic differentiation process

Jia-Lin Liu,Hui-Yu He,Yan-Shi Liu,Mei-Jie Zheng

doi:10.1007/s11033-021-07013-5

Abstract

Tissue engineered bone brings hope to the treatment of bone defects, and the osteogenic differentiation of stem cells is the key link. Inducing osteogenic differentiation of stem cells may be a potential approach to promote bone regeneration. In recent years, lncRNA has been studied in the field increasingly, which is believed can regulate cell cycle, proliferation, metastasis, differentiation and immunity, participating in a variety of physiology and pathology processes. At present, it has been confirmed that certain lncRNAs regulate the osteogenesis of stem cells and take part in mediating signaling pathways including Wnt/β-catenin, MAPK, TGF-β/BMP, and Notch pathways. Here, we provided an overview of lncRNA, reviewed its researches in the osteogenic differentiation of stem cells, emphasized the importance of lncRNA in bone regeneration, and focused on the roles of lncRNA in signaling pathways, in order to make adequate preparations for applying lncRNA to bone tissue Engineering, letting it regulate the osteogenic differentiation of stem cells for bone regeneration.

Highlights

The reconstruction of maxillofacial bone defects provides a challenge in the medical field due to the inherent limitations
Results on in-vitro and invivo experiments indicated that down expression of long non-coding RNA (lncRNA) MEG3 could promote the osteoblast differentiation and fracture healing, it might be mediated by the Wnt/β-catenin signaling pathway [60, 61]
The use of high-throughput sequencing technology combined with bioinformatics analysis has become the mainstream modality for large-scale screening of lncRNAs associated with osteogenic differentiation

Summary

Introduction

The reconstruction of maxillofacial bone defects provides a challenge in the medical field due to the inherent limitations. Studies on lncRNA H19 have demonstrated that its osteogenic effect is to activate the Wnt/βcatenin signaling pathway in combination with miR-141, miR-22 and miR-541-3p [7, 19]. LncRNA MALAT1 up-regulated the expression of Smad4 by sponging miR-204, enhancing the osteogenic differentiation of hAVICs [27].

Results

Conclusion