Abstract
As the main constituent of the largest cellular ribonucleoprotein complex, the evolutionary highly conserved major vault protein (MVP) has been proposed play vital roles in the regeneration of multiple organs. In current study, we use a mvp knockout zebrafish line recently generated to characterize the function of MVP during organ regeneration. We found the regenerative capacity of heart, spinal cord and fin is preserved in mvp knockout zebrafish. Further experiments demonstrated in injured mvp knockout zebrafish, the cell death is enhanced while the transcriptome landscape is largely unchanged. These data showed MVP acts as an anti-apoptotic factor at early phase of injury response while plays a dispensable role in the regenerative programs in zebrafish.
Highlights
The evolutionary highly conserved major vault protein (MVP) is the main constituent of the largest naturally occurring ribonucleoparticles known as vaults (Berger et al, 2009)
To better define the spatiotemporal expression pattern of mvp during regeneration, we generated a mvp:EGFP reporter strain with BAC sequences containing zebrafish mvp (Figures 1A and S1) and evaluated EGFP signal in injured heart, spinal cord and fin
This result is corroborative with the observation that mvp was transactivated at the acute phase of injury response in all three types of tissues analysed
Summary
The evolutionary highly conserved major vault protein (MVP) is the main constituent of the largest naturally occurring ribonucleoparticles known as vaults (Berger et al, 2009). Due to majority of the research was carried out on in vitro systems, physiological role of MVP largely remains unknown. Though an absolute function of cellular vaults has not been determined, several reports suggested these ribonucleoprotein particles might play a role in organ regeneration of zebrafish. Expression analysis revealed after injury, expression of MVP is induced in heart, caudal fin and spinal cord of zebrafish (Ma et al, 2018; Pan et al, 2013; Yoshinari et al, 2009). By using an morpholino-based knock-down approach, researchers observed reduced axonal regrowth after spinal cord transection and proposed MVP supports locomotor recovery and axonal regrowth in adult zebrafish (Pan et al, 2013). While so far the function of MVP on regeneration has not been systematically examined and evaluated by using genetic tools
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