The Major Phyto-Compounds Heptasiloxane, hexadecamethyl- and 1,1-Dimethylethyl 3-Phenyl-2-Propenoate Derived from Indigofera tinctoria Medicinal Flora Tested Against Various Target Medical and Agronomic Pests

Abstract

Objectives: To determine the phytochemical screening and major phytocompounds from Indigofera tinctoria leaf methanol extract as well as ovicidal and larvicidal toxicity towards medical and agronomic pests, Aedes aegypti, Culex quinquefasciatus, Spodoptera litura and Helicoverpa armigera. Methods: In this study, the phytochemical screening was done by prescribed method, finding of phyto-constituents were made by GC-MS analysis, ovicidal and larvicidal toxicity of selected pests were recorded after 24 hrs. post treatment at various concentrations. The mortality was assessed by using probit analysis to calculate LC50/LC90. Findings: By the phytochemical screening, the more numbers of phytochemicals were obtained from methanol extract as well as GC-MS analysis displayed sum of 10 phyto-compounds gained 100% besides two phyto-compounds were major constituents Heptasiloxane, hexadecamethyl- and 1,1-Dimethylethyl 3-Phenyl-2-Propenoate. The phytoproducts of I. tinctoria were produced maximum eggs toxicity around 100% at higher concentration. Similarly, I. tinctoria leaf methanol extract borne major phyto-compounds major constituents Heptasiloxane, hexadecamethyland 1,1-Dimethylethyl 3-Phenyl-2-Propenoate were tested 3rd instar larvae of selected medicinal and agronomic pests with their LC50/ LC90 value were 10.93/18.65 mg/ml, 10.87/18.77 mg/ml, 15.29/27.17 mg/ml, 16.84/29.41mg/ml and 11.16/19.38 mg/ml, 10.43/18.51 mg/ml, 14.57/26.58 mg/ml and 15.61/28.63 mg/ml were recorded on various pests of Ae. aegypti, Cx. quinquefasciatus and S. litura and H. armigera, respectively. Novelty: The statistical analysis of the data clearly indicates that phyto-compounds of I. tinctoria induced outstanding larvallethality were observed on selected pests. Particularly, the identified phytocompounds showed multifold toxicity against the selected pests. Keywords: Indigofera tinctoria; Phytocompound; Targetfauna; Ecosafety; Pesttoxicity