Abstract
ABSTRACTAn improved method is described for the isolation of nuclei from the nonphotosynthetic dinoflagellate Crypthecodinium cohnii (Seligo) Chatton in Grassé. The new method results in an increase in yield from about 13% to 45% or higher, with no loss in purity or activity of endogenous RNA polymerase. Most of this increase in yield is from the ability of 25% Ficoll to cause many cells to shed their walls, and from the stabilizing effect of hexylene glycol during cell disruption. The histone‐like protein, previously undetectable in chromatin from stationary phase cells, can now be demonstrated in chromatin from stationary as well as log phase cells. Preliminary experiments indicate that this protein inhibits transcription.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
