Abstract
BackgroundNasopharyngeal carcinoma (NPC) is a malignant tumor originating from the epithelial cells of the nasopharyngeal mucosa of the head and neck. The role of long non-coding RNA and RNA methylation in NPC has received increasing attention. Therefore, this study aims to investigate the mechanism of lncRNA ZFAS1 in NPC and its relationship with RNA methylation, providing evidence for targeted therapy of NPC.MethodsMicroarray arrays were used to screen the differentially expressed miRNAs in normal tissues and tumor tissues. QRT-PCR was used to quantify ZFAS1, miR-100-3p, ATG10, autophagy and epithelial-mesenchymal transition related genes. The interactive relationship between ZFAS1 and miR-100-3p was verified using dual-luciferase reporter gene assay and RIP assay. CCK-8, transwell and apoptosis were used to detect the occurrence of tumor cells after different treatments. The m6A modification test is used to verify the effect of METTL3 on ZFAS1. BALB/c mice and BALB/c nude mice are used to detect the effects of different treatments on tumor growth and immune escape in vivo.ResultsZFAS1 is upregulated in tumor tissues and NPC cells. N (6)-methyladenosine (m6A) is highly enriched in ZFAS1 and enhances its RNA stability. ZFAS1 is used as an oncogenic lncRNA, which can promote NPC cell proliferation, migration and tumor growth. In terms of mechanism, ZFAS1 up-regulates the expression of ATG10 by competitively adsorbing miR-100-3p and regulates the level of autophagy by inhibiting the PI3K/Akt signaling pathway to promote the proliferation and migration of NPC cells.ConclusionIn short, our study verified the cancer-promoting effect of ZFAS1 in NPC and explained part of the reason for its upregulation. In addition, we confirmed that ZFAS1 can regulate the autophagy level of NPC cells through the PI3K/AKT pathway through miR-100-3p/ATG10 to affect tumor progression.
Highlights
Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from the epithelial cells of the nasopharyngeal mucosa of the head and neck
Effects of ZFAS1 on the proliferation and apoptosis of NPC cells Through the above experiments, we found that the expression of ZFAS1 was up-regulated in tumor tissues and NPC cells
In the detection of cell apoptosis, we found that inhibiting the expression of ZFAS1 in HONE-1 cell could significantly promote the increase in the rate of apoptosis, while overexpression of ZFAS1 in HK-1 cell could significantly inhibit the occurrence of apoptosis. (Fig. 2D, p < 0.05)
Summary
Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from the epithelial cells of the nasopharyngeal mucosa of the head and neck. Nasopharyngeal carcinoma (NPC) is a malignant tumor derived from the epithelial cells of the nasopharyngeal mucosa of the head and neck. Peng et al Infectious Agents and Cancer (2022) 17:1 the treatment of NPC, like most tumors, the stage of the disease is a key factor affecting prognosis and survival [3]. The latest data shows that only early radiotherapy for NPC has the best effect, but as the lesion progresses, local and distant recurrence will affect the 5-year overall survival rate. Finding efficient biomarkers for early diagnosis and in-depth exploration of pathogenic mechanisms, effective development of disease prevention and targeted therapy is the top priority of current research
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