Abstract
Lysobacter capsici AZ78 has considerable potential for biocontrol of phytopathogenic microorganisms. However, lack of information about genetic cues regarding its biological characteristics may slow down its exploitation as a biofungicide. In order to obtain a comprehensive overview of genetic features, the L. capsici AZ78 genome was sequenced, annotated and compared with the phylogenetically related pathogens Stenotrophomonas malthophilia K729a and Xanthomonas campestris pv. campestris ATCC 33913. Whole genome comparison, supported by functional analysis, indicated that L. capsici AZ78 has a larger number of genes responsible for interaction with phytopathogens and environmental stress than S. malthophilia K729a and X. c. pv. campestris ATCC 33913. Genes involved in the production of antibiotics, lytic enzymes and siderophores were specific for L. capsici AZ78, as well as genes involved in resistance to antibiotics, environmental stressors, fungicides and heavy metals. The L. capsici AZ78 genome did not encompass genes involved in infection of humans and plants included in the S. malthophilia K729a and X. c. pv. campestris ATCC 33913 genomes, respectively. The L. capsici AZ78 genome provides a genetic framework for detailed analysis of other L. capsici members and the development of novel biofungicides based on this bacterial strain.
Highlights
Genome sequencing represents an excellent tool for biological characterization of bacterial species; especially in the case of species that have been largely underexplored
The L. capsici AZ78 (Lc AZ78) genome was compared with the genomes of the opportunistic human pathogen Sm K729a and the phytopathogen Xcc ATCC 33913
There are some Lc AZ78-specific genes related to fungicide and antibiotic resistance, such as three genes (AZ78_906, 1192, and 2446) encoding putative Small Multidrug Resistance protein (SMR) proteins and six genes (AZ78_266, 1103, 3068, 3688, 3949, and 4767) encoding putative Major Facilitator Super-family (MFS) proteins (Table 2). Since all these efflux systems can contribute to resistance to antibiotics, we evaluated the sensitivity of Lc AZ78 to several antibiotics in vitro (Table 5)
Summary
Genome sequencing represents an excellent tool for biological characterization of bacterial species; especially in the case of species that have been largely underexplored. Other bacterial genera have been underexplored, as in the case of the genus Lysobacter, which was established in 1978 (Christensen and Cook, 1978). Cythophagales, and several Lysobacter strains were wrongly assigned to Stenotrophomonas and Xanthomonas spp. The increasing number of 16S rDNA gene sequences available in public databases and the polyphasic approach to the identification of bacterial strains has led to an increase in the identification of new Lysobacter species. The genus has expanded to include 37 species (Singh et al, 2015) from the initial four: L. antibioticus, L. brunescens, Lysobacter enzymogenes, and L. gummosus (Christensen and Cook, 1978)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
