Abstract
The investigation of innovative label-free α-amino acids detection methods represents a crucial step for the early diagnosis of several diseases. While 1,8-diazafluoren-9-one (DFO) is known in forensic application because of the fluorescent products by reacting with the amino acids present in the papillary exudate, its application for diagnostic purposes has not been fully investigated. The stabilization of DFO over a transparent substrate allows its complexation with biomolecules for the detection of α-amino acids. In this study, DFO was immobilized into a titanium dioxide (TiO2) matrix for the fluorescence detection of glycine, as a target α-amino acid (a potential marker of the urogenital tract cancers). The DFO/TiO2 composite was characterized by atomic force microscopy, spectroscopic ellipsometry, fluorescence spectroscopy and fluorescence microscopy. The performed fluorescent studies indicate spectacular formation of aggregates at higher concentration. The measurements performed using various fluorescence and microscopic techniques together with the suitable analysis show that the aggregates are able to emit short-lived fluorescence.
Highlights
The aromatic ketone, 1,8-diazafluoren-9-one (DFO) contains heteroatoms in a ring structure
The AFM topography images of DFO/TiO2 thin films with various DFO concentrations are displayed in topography images of DFO/TiO
It was found that the aggregation process of DFO, in the TiO2 matrix, at a sufficiently high concentration, developed rapidly
Summary
The aromatic ketone, 1,8-diazafluoren-9-one (DFO) contains heteroatoms (nitrogen and oxygen) in a ring structure. One of the goals of this work was to study basic aggregate properties in the titanium dioxide thin film, which is important for its potential application, since the aggregation process can shorten the fluorescence decays and lifetimes of monomers. This is the case of many other molecular systems in which aggregates are formed [2,3,4,5,6], for example in some medical applications [7,8]. The affinity of DFO with TiO2 and the versatility of the sol–gel technique allow designing and developing an organic–inorganic framework exhibiting the desired optical/chemical properties
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