Abstract
BackgroundVerticillium wilt caused by the fungus Verticillium dahliae race 1 is among the top disease concerns for lettuce in the Salinas and Pajaro Valleys of coastal central California. Resistance of lettuce against V. dahliae race 1 was previously mapped to the single dominant Verticillium resistance 1 (Vr1) locus. Lines of tomato resistant to race 1 are known to contain the closely linked Ve1 and Ve2 genes that encode receptor-like proteins with extracellular leucine-rich repeats; the Ve1 and Ve2 proteins act antagonistically to provide resistance against V. dahliae race 1. The Vr1 locus in lettuce contains a cluster of several genes with sequence similarity to the tomato Ve genes. We used genome sequencing and/or PCR screening along with pathogenicity assays of 152 accessions of lettuce to investigate allelic diversity and its relationship to race 1 resistance in lettuce.ResultsThis approach identified a total of four Ve genes: LsVe1, LsVe2, LsVe3, and LsVe4. The majority of accessions, however, contained a combination of only three of these LsVe genes clustered on chromosomal linkage group 9 (within ~ 25 kb in the resistant cultivar La Brillante and within ~ 127 kb in the susceptible cultivar Salinas).ConclusionsA single allele, LsVe1L, was present in all resistant accessions and absent in all susceptible accessions. This allele can be used as a molecular marker for V. dahliae race 1 resistance in lettuce. A PCR assay for rapid detection of race 1 resistance in lettuce was designed based on nucleotide polymorphisms. Application of this assay allows identification of resistant genotypes in early stages of plant development or at seed-level without time- and labor-intensive testing in the field.
Highlights
Verticillium wilt caused by the fungus Verticillium dahliae race 1 is among the top disease concerns for lettuce in the Salinas and Pajaro Valleys of coastal central California
One of the top disease concerns for lettuce in the area is Verticillium wilt caused by the fungus Verticillium dahliae [2, 3], which is a soilborne pathogen with a wide host range that includes artichoke, cotton, eggplant, hops, potato, sunflower, tobacco, and tomato [4
Ve genes encode receptor-like proteins (RLPs) with extracellular leucine-rich repeats [9, 10]; such Receptor-like proteins (RLP) are widespread in land plants [11]
Summary
Verticillium wilt caused by the fungus Verticillium dahliae race 1 is among the top disease concerns for lettuce in the Salinas and Pajaro Valleys of coastal central California. Lines of tomato resistant to race 1 are known to contain the closely linked Ve1 and Ve2 genes that encode receptor-like proteins with extracellular leucine-rich repeats; the Ve1 and Ve2 proteins act antagonistically to provide resistance against V. dahliae race 1. One of the top disease concerns for lettuce in the area is Verticillium wilt caused by the fungus Verticillium dahliae [2, 3], which is a soilborne pathogen with a wide host range that includes artichoke, cotton, eggplant, hops, potato, sunflower, tobacco, and tomato [4, 5]. Several Ve paralogs confer resistance in otherwise V. dahliae-susceptible species including cotton [13], potato [14, 15], hops, and wild eggplant [11], but it is unknown whether they function analogously to the tomato Ve genes in conferring V. dahliae race 1
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