The lowest diploid number in Testudines: Banding patterns, telomeric and 45S rDNA FISH in Peltocephalus dumerilianus, 2n = 26 and FN = 52 (Pleurodira, Podocnemididae)

Karen Ventura,Yatiyo Yonenag-Yassuda,Miguel T Rodrigues,Camila N Moreira,Renata Moretti

doi:10.1590/s1415-47572014000100011

Karen Ventura, Yatiyo Yonenag-Yassuda + Show 3 more

Open Access

https://doi.org/10.1590/s1415-47572014000100011

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Journal: Genetics and Molecular Biology	Publication Date: Feb 28, 2013
Citations: 11	License type: cc-by

Affiliation: Universidade de São Paulo

Abstract

The karyotype of the big-headed Amazon River turtle, Peltocephalus dumerilianus, is characterized based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas, Pará State, Brazil (1°30' S, 56°34' W). Here we present the first results on GTG and CBG-banding patterns, Ag-NOR staining and FISH, with telomeric and 45S rDNA sequences as probes. A cytogenetic comparison with related Podocnemidae is also provided.

Highlights

Based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas (1°30’ S, 56°34’ W), Amazonian forest of Pará State, Brazil, the karyotype of P. dumerillianus was characterized for the first time using routine differential techniques, such as GTG, CBGbanding and Ag-NOR staining (Seabright, 1971; Sumner, 1972; Howell and Black, 1980, with modifications) and molecular tools employing FISH with telomeric and 45S rDNA sequences as probes in metaphases obtained from in vivo bone marrow preparations (Ford and Hamerton, 1956)
The FISH procedures for the 45S rDNA were followed the method adapted by Cabral-de-Mello et al (2010)
The 45S rDNA probes were biotinylated by nick-translation (Invitrogen, San Diego, CA, USA) and detected with avidin-Cy3 (Life-Technologies)

Summary

Introduction

Based on a sample of seven juveniles from Reserva Biológica do Rio Trombetas (1°30’ S, 56°34’ W), Amazonian forest of Pará State, Brazil, the karyotype of P. dumerillianus was characterized for the first time using routine differential techniques, such as GTG, CBGbanding and Ag-NOR staining (Seabright, 1971; Sumner, 1972; Howell and Black, 1980, with modifications) and molecular tools employing FISH with telomeric and 45S rDNA sequences as probes in metaphases obtained from in vivo bone marrow preparations (Ford and Hamerton, 1956). On the proximal region of chromosome 1p (Ortiz et al, 2005; Fantin and Monjeló, 2011). The FISH procedures for the 45S rDNA were followed the method adapted by Cabral-de-Mello et al (2010).

Results

Conclusion