The Low Expression Level of Pokeweed Antiviral Protein (PAP) Gene inEscherichia coliby the InduciblelacPromoter Is Due to Inefficient Transcription and Translation and Not to the Toxicity of the PAP

Abstract

Pokeweed (Phytolacca americana) antiviral protein (PAP) is a glycosidase which inactivates both eukaryotic and prokaryotic ribosomes. Due to this activity the wild-type PAP gene encoding mature protein has not so far been expressed inEscherichia coli.In spite of the ribosome impairing activity of the pre-PAP (containing two signal peptides at both termini) on bacterial ribosomesin vitro,the full-length PAP gene has been expressed successfully, although at a low level inE. coliunder an induciblelacpromoter. In this study we show that the full-length PAP gene, but not the PAP gene devoid of the N-terminal signal peptide codons, can be expressed constitutively inE. colicells to produce a much higher yield as compared with the inducible expression. The full-length PAP is biologically active and it accumulates as inclusion bodies in bacterial cytoplasm. RNA analysis together with protein measurements show that the PAP gene is poorly transcribed and the PAP mRNA is poorly translated when alacoperator sequence is placed in front of the Shine/Dalgarno (SD) sequence. Nucleotide folding analysis of the 5′ untranslated mRNA revealed that the SD sequence in the presence of alacoperator is involved in a stable secondary structure, whereas it is more relaxed in the mRNA transcribed from the constitutive vector. These results provide evidence that the low expression level of full-length PAP gene is due to inefficient transcription and translation but not to the toxicity of the expressed PAP.