Abstract
Various nitrate concentrations (0, 1, 2, 4, 8, 20, 50 mol m −3 ) were applied at weekly intervals for 10 weeks to the caesalpinioid legume Chamaecrista fasciculata. Microscopic techniques and general growth studies showed that nitrate affected both the plant and its rhizobial symbiosis. As the nitrate concentration was increased, nodule structure became increasingly disrupted even though nitrate remained limiting to plant growth until 8 mol m −3 . Poly-β-hydroxybutyrate (PHB) was observed using transmission electron microscopy; as nitrate increased from 0 to 2 mol m −3 , the PHB stores were utilized
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