The long-term effects of crude oil on microbial processes in subarctic marine sediments

Abstract

Subarctic marine sediments were exposed to fresh and ‘weathered’ crude oil from Cook Inlet, Alaska. The crude oil was thoroughly mixed with the sediment at various concentrations and some of the oil-sediment mixtures were placed on top of unamended sediments. Both sediments with added oil and those overlain by oiled sediments were investigated. These sediments were placed either in trays or aquaria. The trays were incubated in situ near the site from which the sediments were originally collected. The aquaria were maintained at simulated in situ conditions by maintaining a continuous flow of fresh seawater through them. The sediments were exposed to crude oil for periods up to 1.5 years. Sediments exposed to 50‰ fresh crude oil showed significant decreases in nitrogen fixation and denitrification rates and redox potentials. Also observed were increases in CO 2 production rates and methane concentrations. These same changes were observed in sediments exposed to 1‰. When ‘weathered’ crude oil was added to the sediments, the same changes were observed except there was no reduction in nitrogen fixation activity. In most cases, the observed effects were less marked when the sediments were amended with ‘weathered’ crude oil than with the same concentration of fresh crude oil. Untreated sediments that were overlain with treated sediments showed the same changes as those sediments that were thoroughly mixed with crude oil. The presence of fresh crude oil at 50‰ essentially eliminated burrowing activity of the benthic infauna. Under these conditions, there was an accumulation of detritus particles on the oiled sediments that was not present in the untreated controls.