Abstract

The distribution of total, acid-soluble, and phospholipid phosphorus to the egg, polar lobe, lobeless egg, AB and CD blastomeres was determined. The concentration of all three groups of phosphorus compounds was significantly greater in the polar lobe and CD blastomere than in the egg or any of the other isolates studied. The distribution of the acid-soluble phosphorus is correlated with the distribution of yolk to the polar lobe and blastomeres. The problem of choosing a reference unit for calculating the concentration of the acid-soluble phosphorus is discussed. The phospholipids were found to be equally distributed between the yolk and yolk-free cytoplasm, and as a result the concentration of the phospholipids could be reliably calculated. The concentration of phospholipids, as measured by their phosphorus content, in the polar lobe exceeded that of the AB blastomere by a factor of 1.90; the concentration of phospholipids in the CD blastomere was 1.27 times greater than in the AB blastomere.

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