Abstract
1. 1. It has been possible to demonstrate that diaminopimelic acid in the peptidoglycan of Bacillus megaterium NCTC 7581 is α-linked. One method utilized the photolability of the dinitrophenylamino group when such a group is linked directly to a carbon atom bearing a carboxyl group. Thus after irradiation of dinitrophenylated peptidoglycan and acid hydrolysis, only a trace of mono-(dinitrophenyl) diaminopimelic acid remained. When irradiated peptidoglycan was oxidized and hydrolyzed, the expected degradation product of diaminopimelic acid, α-aminoadipic acid was detected and estimated. 2. 2. The Lossen rearrangement was used to demonstrate that 75% of both glutamic acid and diaminopimelic acid residues in the peptidoglycan have one free carboxyl group. α-Aminoadipic acid was obtained from Lossen rearrangement of dinitrophenylated peptidoglycan after subsequent oxidation and hydrolysis, thus confirming that the diaminopimelic acid residues are α-linked.
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