Abstract
A limit dextrinase has been isolated from broad-bean flour and purified 800-fold. The enzyme readily hydrolysed branched α-dextrins containing maltosyl or maltotriosyl side-chains, pullulan, and amylopectin β-limit dextrin. Glycogen β-limit dextrins and amylopectin were slowly hydrolysed, but glycogens were barely susceptible to hydrolysis. There was no evidence for the presence of a second debranching enzyme in broad beans.
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