Abstract
Photoreceptors of dissociated Drosophila retinae were loaded with the fluorescent Ca 2+ indicators, fluo-3 and Calcium Green-5N. In fluo-3-loaded, wild-type photoreceptors, a rapid increase in fluorescence (Ca 2+ signal) accompanied the light-evoked inward current. Removal of extracellular Ca 2+ greatly reduced the Ca 2+ signal, indicating Ca 2+ influx as its major cause. In Calcium Green-5N-loaded trp mutants, which lack a large fraction of the Ca 2+ permeability underlying the light-evoked inward current, the Ca 2+ signal was smaller relative to wild-type photoreceptors. Fluo-3-loaded norpA mutant photoreceptors, which lack a light-activated phospholipase C, generated no light-evoked inward current and no Ca 2+ signal. The phosphoinositide pathway therefore appears necessary for both excitation and changes in cytosolic free Ca 2+ concentration.
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