The life-cycle of Gorgocephalus yaaji Bray & Cribb, 2005 (Digenea: Gorgocephalidae) with a review of the first intermediate hosts for the superfamily Lepocreadioidea Odhner, 1905.

Abstract

Using novel molecular and morphological data we elucidated the life-cycle of Gorgocephalus yaaji Bray & Cribb, 2005 from off Lizard Island, on the northern Great Barrier Reef, Australia. ITS2 rDNA sequences generated for larval trematodes from the infected snail species Echinolittorina austrotrochoides Reid (Littorinidae) were identical to those from adult G.yaaji from the fish Kyphosus cinerascens (Forsskål) (Kyphosidae). Cercariae develop in rediae in E. austrotrochoides, emerge from the snail, encyst on algae as metacercariae, and are inferred to then be consumed by the herbivorous definitive fish host, K.cinerascens. In addition, we generated the first ITS2 rDNA sequences for a gorgocephalid previously reported from the littorind gastropod Austrolittorina unifasciata Gray. Although infections previously reported from A.unifasciata were the first larval gorgocephalids characterised, this study is the first to connect an intramolluscan infection to a sexual adult. In light of the new life-cycle information, a review of mollusc associations for the digenean superfamily Lepocreadioidea was performed, highlighting gaps in the knowledge and revealing patterns of host-parasite association. We find that distinct patterns of first intermediate host association are discernible for three lepocreadioid lineages: the Aephnidiogenidae Yamaguti, 1934, Gorgocephalidae Manter, 1966, and the Lepocreadiidae Odhner, 1905. However, the evolutionary origin for these patterns of host association remains unclear.