Abstract
The levels of yield and purity of genomic DNA from five tomato cultivars subjected to two DNA extraction techniques
Highlights
DNA extraction is a routine step in many biological studies including molecular identification, phylogenetic inference, genetics, and genomics
DNA can interfere polymerase chain reaction (PCR) reactions, for example polyphenolic compounds, polysaccharides and RNA (Krishna et al, 2012) and these can hamper the isolation of good quality DNA (Arif et al, 2010)
When Dellaporta et al method was used good quality DNA was extracted from Santana, F1-Mongal, Kerewa, Tyre-type and Pure-water cultivars which showed that contaminants were not present
Summary
DNA extraction is a routine step in many biological studies including molecular identification, phylogenetic inference, genetics, and genomics. Ecology of southwestern Nigeria is very favorable for tomato production It is an economic fruit vegetable for tomato growers, consumed fresh and used to manufacture a wide range of processed products. One of the most challenging is bacterial wilt caused by Ralstonia solanacearum. This disease can constitute up to 100% yield loss in endemic areas (Popoola et al, 2015). Breeding a resistant variety using an updated gamplasm as a donor typically requires a series of backcrosses to the cultivated recurrent parent to combing desirable characteristics. The application of molecular breeding through extraction
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