The levels of CXCR3 mRNA expression on peripheral blood monocytes of patients with primary biliary cirrhosis

Abstract

Objectlve To detect the expression of CXCR3 mRNA in peripheral blood mononuclear cells (PBMC) in patients with primary biliary cirrhosis (PBC) ,and explore its relationship with activity of PBC.Methods Reverse transcription-real time quantitative polymerase chain reaction (FQ-RT-PCR) was used to examine CXCR3 mRNA expression in peripheral blood monocytes of 29 cases of PBC in active stage. 30 cases in stable stage,20 cases in CTD,and 30 healthy controls.Results The mean level of CXCR3 mRNA expression of PBc inactive stage (△Ct=5.41±2.69) Was higher than that of stable stage (△Ct=7.77±2.74,t=3.39,P＜0.01),CTD(△Ct=7.24±2.75,t=2.53,P＜0.01),and healthy controls (△Ct =7.16±2.76,t=2.45,P＜0.01).There was no significant difference of expression levels of CXCR3 mRNA among stable stage PBC patients,CTD diseases patients and healthy controls (P＞0.05).Conclusion This study indicated that the CXCR3 mRNA expression levels of PBMC is significantly elevated in patients with active PBC.and it could be implicated in pathogenesis and activity of disease. Key words: Liver cirrhosis biliary; Leukocytes,mononuclear; Chemokines,CX3C; Receptores,chemokine; Reverse transcriptase polymerase chain reaction