The level and clinical significance of serum anti-lysosomal associated membrane protein-2 antibody in patients with anti-neutrophil cytoplasmic antibodies-associated vasculitis

Abstract

Objective To explore the clinical significance of serum anti-lysosomal associated membrane protein-2(LAMP-2)antibody in the pathogenesis of anti-neutrophil cytoplasmic antibodies(ANCA)-associated vasculitis(AASV)by investigating the relationship of its levels and AASV. Methods Sera from twenty patients with AASV and twenty healthy controls were collected. Serum anti-LAMP-2 antibody was detected using commercial enzyme linked immunosorbent assay(ELISA)kits. Anti-LAMP-2 antibody levels between the two groups were assessed using the t test, the correlation between anti-LAMP-2 antibody levels and erythrocyte sedimentation rate(ESR), C-reactive protein(CRP)was assessed by Spearman's rank correlation test, the correlation between anti-LAMP-2 antibody levels and the Birmingham vasculitis activity score(BVAS), hemoglobin(Hb), albumin(Alb)was assessed by Pearson's rank correlation test. Results ① The serum level of anti-LAMP-2 antibody in patients with AASV[(3 714±1 446)pg/ml]was higher than that in the healthy controls[(174土43)pg/ml](t=10.94, P 0.05).② The level of anti-LAMP-2 antibody in AASV was positively correlated with BVAS(r=0.669 9, P 0.05). Conclusion ① Anti-LAMP-2 antibody is involved in the pathogenesis of AASV.② Anti-LAMP-2 antibody is correlated with the activity of AASV, it may be an indicator of AASV disease activity. Key words: Antibody; Lysosomal associated membrane protein-2; Anti-neutrophil cytoplasmic antibodies-associated vasculitis