Abstract
We report that the results of our study indicate that Lactobacillus brevis 47 f strain isolated from the faeces of a healthy individual prevents the manifestations of experimental mucositis induced by treatment of Balb/c mice with the anticancer drug 5-fluorouracil (5 FU; 100 mg/kg i.p. × 3 days). The presence of damage to the intestine and the colon was determined by a morphometric analysis of specimens including the height of villi, the amount of goblet cells and infiltrating mononuclear cells, and the expression of the proliferative Ki-67 antigen. Changes in the lipid peroxidation in the blood and the intestine were determined by severalfold increase of the concentration of malonic dialdehyde. Oral administration of L. brevis 47 f strain prior to 5 FU decreased the drug-induced morphological and biochemical changes to their respective physiological levels; the ability of intestinal epitheliocytes to express Ki-67 was partially restored. These effects of L. brevis 47 f strain were more pronounced or similar to those of the reference compound Rebamipid, a quinoline derivative known to protect the gut from drug-induced toxicity. Thus, the new lactobacilli strain attenuates the severity of 5 FU-induced enteropathy.
Highlights
Reactive oxygen species (ROS) represent a key mechanism of regulation of a plethora of physiological processes
In this study we investigated the ability of L. brevis 47 f to protect the murine intestine from 5 FU induced mucositis
Gross anatomical examination revealed no abnormalities in intact mice, as well as in L. brevis 47 f or Rebamipid cohorts
Summary
Reactive oxygen species (ROS) represent a key mechanism of regulation of a plethora of physiological processes. This evolutionarily-conserved mechanism regulates metabolism in all organisms, from Protozoa to mammals [1,2]. Lactobacilli, have long been recognized as a natural source of potentially useful agents. These species can be advantageous over chemical compounds due to their safety and biocompatibility. Many lactobacilli display antioxidant activity that has been determined in bacterial cultures and in animal models. The antioxidant properties, together with immunostimulatory and metabolic activities, and antagonism against pathogenic microorganisms, make lactobacilli attractive therapeutic tools in clinical situations
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