The Labeling Efficiency of Invitro F-18 Fluorodeoxyglucose-Leukocyte

Abstract

Introduction: In our study, we aimed to investigate the effect of leukocyte incubation temperature, F-18 FDG dose and the use of insulin in the process on leukocyte labeling efficiency. Methods: The study was conducted in the nuclear medicine department of Pamukkale University Medical Faculty between April and October 2019 and is a prospective study.. Leukocytes were isolated from blood from 24 volunteers (age ranges 25-55 years) with fasting blood glucose of 80-120 mg/dl. Groups were incubated at 37°C or room temperature and labeled with F-18 FDG (2mCi/10 mCi). Each group was divided into two groups as insulin added and not added. Bound and free component activity were measured separately with the dose calibrator. Results: There was no statistically significant difference between insulin-free and insulin-supplemented group (39.5±0.072%; 40.2±0.073, respectively) (p>0,05). In the insulin free group, the percentage of labeling of high-dose F-18 FDG (10 mCi) was found to be significantly higher than low-dose F-18 FDG (2 mCi) (p=0.049), whereas in the insulin-treated group, there was no significant difference between low-dose and high-dose F-18 FDG addition (p=0.09). It was found that the percentage of labeling was statistically higher in the 37˚C incubated insulin added (p=0.036) and non-insulin added (p=0.042) groups compared to the groups that were incubated at room temperature. Conclusion: Leukocyte labeled with F-18 FDG is a method that can be used in the diagnosis of infection. High temperature incubation and high dose F-18 FDG marking increases the labeling efficiency, while insulin administration does not alter the labeling efficiency Key words: F-18 FDG, leukocyte labeling, insulin, infection imaging Special Issue of Health Sciences DOI: 10.7176/JSTR/6-03-18