Abstract
The L5178Y/TK+ ➙ TK− assay is one of several recognized mammalian cell systems for estimating the genotoxic potential of environmental or commercial chemicals. In a typical test application, thymidine kinase- competent (TK+) 3.7.2C L5178Y cells are exposed to five or more different concentrations of the test substance for 1–4 hr, maintained 48–72 hr in growth medium, then cloned in soft-agar medium in a single-step selection process to detect mutagen-induced thymidine kinase-deficient (TK−) variants. Independent validation studies(11,15,30) have demonstrated that this assay is sensitive to more than 20 known genotoxic chemicals. This short-term mutagenesis assay is a good predictor of mammalian carcinogenicity, with few “false positive” results in those studies where noncarcinogens were tested.(8)
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