The Krüppel-like factor 6 represses proliferation and invasion ofcutaneous malignant melanoma.

Abstract

Cutaneous carcinoma is one ofthemost common neoplasm tumors in theWest. Its incidence rate is one ofthefastest growing tumors in China. TheKrüppel-like factor 6 (KLF6) is alatent tumor suppressor. Decreased KLF6 is related to theoccurrence and progression ofmany cancers in human. Our previous studies have demonstrated that KLF6 was down-regulation in cutaneous malignant melanoma (CMM), and was significant correlated with ulcer, lymph node metastasis and clinical stage, suggesting that KLF6 loss is expected to become abiological indicator ofpoor prognosis in CMM patients. In this research, we would further study thefeatures ofKLF6 in themalignant progression ofCMM. Theexpression ofKLF6 was up-regulated by lentivirus infection containing KLF6, and short hairpin RNA (shRNA) was used for knockdown ofKLF6 in CMM cells. Western blot, RT-qpcr, CCK8 assay, transwell migration assays, wound healing assay and flow cytometry were used to test therole ofKLF6 in theCMM. We found that reduced expression ofKLF6 significantly enhanced proliferation, migration and invasion. Moreover, KLF6 induced CMM cell apoptosis and G1 cycle arrest. Thedecreased KLF6 expression is expected to be abiological indicator ofpoor prognosis in CMM patients.